Journal: Cell Cycle

Article Title: Identifying the hub gene in gastric cancer by bioinformatics analysis and in vitro experiments

doi: 10.1080/15384101.2020.1749789

Figure Lengend Snippet: Enrichment plots from GSEA. GSEA results showing that calcium signaling pathway, cell adhesion molecules, cytokine receptor interaction, dilated cardiomyopathy, ECM receptor interaction, focal adhesion, hedgehog signaling pathway, hematopoietic cell lineage, and hypertrophic cardiomyopathy were significantly enriched in SERPINH1 highly-expressed human GC tissues.

Article Snippet: An empty plasmid served as a negative control (scramble). siRNAs designed to specifically silence Serpinh1 was purchased from GenePharma (Shanghai, China).

Techniques:

Journal: Cell Cycle

Article Title: Identifying the hub gene in gastric cancer by bioinformatics analysis and in vitro experiments

doi: 10.1080/15384101.2020.1749789

Figure Lengend Snippet: Correlation of SERPINH1 expression with immune infiltration level in STAD (stomach adenocarcinoma). SERPINH1 expression is significantly negatively related to B cells and has a very weak correlation with macrophages and dendritic cell infiltration levels in STAD. But SERPINH1 expression has no significant correlations with tumor purity and infiltrating levels of CD8 + T cells, CD4 + T cells, and neutrophils in STAD.

Article Snippet: An empty plasmid served as a negative control (scramble). siRNAs designed to specifically silence Serpinh1 was purchased from GenePharma (Shanghai, China).

Techniques: Expressing

Journal: Cell Cycle

Article Title: Identifying the hub gene in gastric cancer by bioinformatics analysis and in vitro experiments

doi: 10.1080/15384101.2020.1749789

Figure Lengend Snippet: SERPINH1 expression is upregulated in GC. (a) and (b) Western blot analysis showed that the SERPINH1 expression level in matched GC tissues (t) and adjacent non-tumor tissues (n), and in normal gastric epithelial and GC cell lines. GAPDH was used as a loading control. (c) Representative immunohistochemistry staining results revealed the protein level expression of SERPINH1 in GC and normal tissues. *P < 0.05.

Article Snippet: An empty plasmid served as a negative control (scramble). siRNAs designed to specifically silence Serpinh1 was purchased from GenePharma (Shanghai, China).

Techniques: Expressing, Western Blot, Control, Immunohistochemistry, Staining

Journal: Cell Cycle

Article Title: Identifying the hub gene in gastric cancer by bioinformatics analysis and in vitro experiments

doi: 10.1080/15384101.2020.1749789

Figure Lengend Snippet: SERPINH1 promotes cell proliferation, colony formation and migration in vitro. (a) The SERPINH1 protein level is increased after overexpression of SERPINH1 in BGC823 cells and decreased after knockdown of SERPINH1 in MKN45 cells. (b) Ectopic expression of SERPINH1 stimulates colony formation in BGC823 cells. Knockdown of SERPINH1 expression inhibits colony formation in MKN45 cells. (c) Ectopic expression of SERPINH1 promotes cell migration in BGC823 cells as demonstrated by transwell assays. Knockdown of SERPINH1 expression inhibits cell migration in MKN45 cells, Original magnification ×100. (d) Ectopic expression of SERPINH1 promotes cell proliferation in BGC823 cells whereas knockdown of SERPINH1 inhibits cell proliferation in MKN45 cells as determined by MTT assays. *P < 0.05.

Article Snippet: An empty plasmid served as a negative control (scramble). siRNAs designed to specifically silence Serpinh1 was purchased from GenePharma (Shanghai, China).

Techniques: Migration, In Vitro, Over Expression, Knockdown, Expressing

Journal: Cell Cycle

Article Title: Identifying the hub gene in gastric cancer by bioinformatics analysis and in vitro experiments

doi: 10.1080/15384101.2020.1749789

Figure Lengend Snippet: SERPINH1 influences the cell cycle progression and reduced the apoptotic rate by using flow cytometry. (a) Cell cycle detected by flow cytometry in BGC823 or MKN45 cells 48 h after transfection with pcDNA3.1 (+)-SERPINH1 or si-SERPINH1, respectively. Histogram represented the percentage of cells in G1, S and G2 cell-cycle phases. (b) Cell apoptosis was detected by Annexin-V/PI with flow cytometry in BGC823 or MKN45 cells 48 h after transfection with pcDNA3.1 (+)-SERPINH1 or si-SERPINH1, respectively. The apoptotic evaluation was calculated by the percentage of apoptotic cell number in total cell number. *P < 0.05.

Article Snippet: An empty plasmid served as a negative control (scramble). siRNAs designed to specifically silence Serpinh1 was purchased from GenePharma (Shanghai, China).

Techniques: Flow Cytometry, Transfection